크로마토그래피 원리의 큰 틀도 마찬가지로 두 상에 대한 분배 차이를 이용하여 분석물을 분리, 정제할 수 있습니다. 다만 크로마토그래피에서 두 개의 상은 하나는 고정하고 다른 하나는 일정 방향으로 이동시켜 사용합니다.
Ion-exchange: Separates billed molecules based mostly on their own conversation with billed useful groups around the stationary section.
-hydroxybenzoic acid elutes extra little by little. While we could solve absolutely both of these solutes employing mobile phase that may be 16% v/v acetonitrile, we can not take care of them In case the cellular phase is ten% tetrahydrofuran.
Transforming the cellular section’s composition as the separation progresses is 1 Option to this problem. For any reversed-phase separation we use an initial cellular period that's additional polar. Since the separation progresses, we modify the composition of cellular phase to ensure it turns into significantly less polar (see Determine 12.five.six
are created by reacting the silica particles with the organochlorosilane of the final form Si(CH3)2RCl, wherever R can be an alkyl or substituted alkyl team.
. The working pump and also the equilibrating pump Just about every Use a piston whose back and forth motion maintains a continuing stream level of approximately a number of mL/min and offers the high output stress necessary to thrust the mobile stage from the chromatographic column.
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The operating tension within an HPLC is sufficiently high that we cannot inject the sample into the mobile phase by inserting a syringe by way of a septum, as is possible in gasoline chromatography. As a substitute, we inject the sample employing a loop injector
The data acquisition system information and processes the signals from your detector, permitting for your development of chromatograms as well as the quantification of compounds.
원하는 분석 결과를 얻기 위해서는 컬럼도 충분히 고려하고 선택하는 것이 좋습니다.
The stationary phase is normally a solid help packed within a column, whereas the cellular stage is often a liquid or a combination of liquids.
, for instance, exhibits retention occasions for 4 weak acids in two cell phases with just about identical values for (P^ key ). Even though the purchase of elution is identical for both equally cellular phases, each solute’s retention time is impacted differently by the selection of natural and organic solvent.
, for example, has two click here cellular section reservoirs which are employed for an isocratic elution or simply a gradient elution by drawing solvents from a single or equally reservoirs.
The selection to start with acetonitrile is arbitrary—we can easily just as simply select to start with methanol or with tetrahydrofuran.